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Marcello Melone


1998-present: Assistant Professor, Department of Experimental and Clinical Medicine, Section of Physiology, Università Politecnica delle Marche, School of Medicine, Ancona, Italy.

2012-present: Researcher, Center for Neurobiology of Aging, INRCA IRCCS, Ancona, Italy.


1994: M.D. Degree (cum laude); University of Ancona, School of Medicine, Ancona, Italy
1998: Specialist in Psychiatry (cum laude); University of Ancona, School of Medicine, Ancona, Italy.

Professional experience:
1991-1994: Undergraduate student; Institute of Human Physiology
1994-1998: Resident; Institute of Human Physiology and Institute of Psychiatry, University of Ancona, School of Medicine, Ancona, Italy
2003-2004: Post-doctoral fellow; Department of Cell and Developmental Biology, Neuroscience Center, University of North Carolina, Chapel Hill 27599 (USA)

Gulisano W, Melone M, Li Puma DD, Tropea MR, Palmeri A, Arancio O, Grassi C, Conti F, Puzzo D (2018) The effect of amyloid-β peptide on synaptic plasticity and memory is influenced by different isoforms, concentrations and aggregation status. Neurobiology of Aging 71: 51-60. doi.org/10.1016/j.neurobiolaging.2018.06.025.

Melone M, Ciriachi C, Pietrobon D, Conti F (2018) Heterogeneity of astrocytic and neuronal GLT-1, as revealed by its co-localization with Na+/K+-ATPase alfa isoforms at cortical excitatory synapses. Cerebral Cortex, in press. doi: 10.1093/cercor/bhy203

Mariotti L, Losi G, Lia A, Melone M, Chiavegato A, Gomez-Gonzalo M, Sessolo M, Bovetti S, Forli A, Zonta M, Requie Marcon I, Pugliese A, Viollet C, Bettler B, Fellin T, Conti F, Carmignoto G (2018). Interneuron-specific signalling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes. Nature Communications, 9:82. doi: 10.1038/s41467-017-02642-6

Fattorini G, Melone M, Sánchez-Gómez MV, Arellano RO, Bassi S, Matute C, Conti F (2017). GAT-1 mediated GABA uptake in rat oligodendrocytes. Glia 65:514-522. doi: 10.1002/glia.23108

Capuani C, Melone M, Tottene A, Bragina L, Crivellaro G, Santello M, Casari G, Conti F, Pietrobon D (2016) Defective glutamate and K+ clearence by cortical astrocytes in familial hemiplegic migraine type 2. EMBO Molecular Medicine 8:967-986. doi: 10.15252/emmm.201505944.

Melone M, Ciappelloni S, Conti F (2014) Plasma membrane transporters GAT-1 and GAT-3 contribute to heterogeneity of GABAergic synapses in neocortex. Frontiers in Neuroanatomy doi: 10.3389/fnana.2014.00071.

Melone M, Ciappelloni S, Conti F (2013) A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex. Brain Structure and Function doi: 10.1007/s00429-013-0690-8.

Milanese M, Giribaldi F, Melone M, Bonifacino T, Vergani L, Musante I, Rossi PIA, Voci A, Conti F, Puliti A, Bonanno G (2013) Knocking-down metabotropic glutamate 1 receptors improves survival and disease progression in the SOD1G93A mouse model of amyotrophic lateral sclerosis. Neurobiology of Disease doi: 10.1016/j.nbd.2013.11.006.

Bragina L, Fattorini G, Giovedí S, Melone M, Bosco F, Benfenati F and Conti F (2012) Analysis of synaptotagmin, SV2, and Rab3 expression in cortical glutamatergic and GABAergic axon terminals. Frontiers in Cellular Neuroscience 5:32.doi:10.3389/fncel.2011.00032.

Conti F, Melone M, Fattorini G, Bragina L, Ciappelloni S (2011) A role for GAT-1 in presynaptic GABA homeostasis? Frontiers in Cellular Neuroscience 5:2. doi: 10.3389/fncel.2011.00002

Melone M, Bellesi M, Ducati A, Iacoangeli M, Conti F (2011) Cellular and synaptic localization of EAAT2a in human cerebral cortex. Frontiers in Neuroanatomy doi: 10.3389/fnana.2010.00151

de Vivo L, Melone M, Bucci G, Rothstein JD, Conti F (2010) Quantitative analysis of EAAT4 promoter activity in neurons and astrocytes of mouse somatic sensory cortex. Neuroscience Letters doi:10.1016/j.neulet.2010.03.003

de Vivo L, Melone M, Rothstein JD, Conti F (2010) GLT-1 promoter activity in astrocytes and neurons of mouse hippocampus and somatic sensory cortex. Frontiers in Neuroanatomy 3:31. doi:10.3389/neuro.05.031.2009

Omrani A, Melone M, Bellesi M, Safiulina V, Aida T, Tanaka K, Cherubini E, Conti F (2009) Up-regulation of GLT-1 severely impairs LTD at mossy fibre-CA3 synapses. Journal of Physiology 587:4575-4588

Fattorini G, Verderio C, Melone M, Giovedì S, Benfenati F, Matteoli M , Conti  F (2009) VGLUT1 and VGAT are sorted to the same populations of synaptic vesicles in subsets of cortical axon terminals. Journal of Neurochemistry 110:1538-46

Bellesi M, Melone M, Gubbini A, Battistacci S, Conti F (2009) GLT-1 up-regulation impairs prepulse inhibition of the startle reflex in adult rats. Glia 57:703-713

Melone M, Bellesi M, Gubbini A, Conti F (2009) GLT-1 up-regulation enhances the effect of PCP on prepulse inhibition of the startle reflex in adult rats. Schizophrenia Research 109:196-197

Fattorini G, Bragina L, Candiracci C, Melone M, Cozzi A, Pellegrini-Giampietro DE, Conti F (2009) Acute phencyclidine administration reduces extracellular glutamate levels and the expression of synaptophysin and SNAP-25 in rat frontal cortex. Schizophrenia  Research 108:288-289.

Melone M, Bellesi M, Conti F (2009) Synaptic localization of GLT-1a in the rat somatic sensory cortex. Glia 57:108-117.

Fattorini G, Melone M, Bragina L, Candiracci C, Cozzi A, Pellegrini-Giampietro DE, Torres-Ramos M, Pérez-Samartín A, Matute C, Conti F (2008) GLT-1 expression and Glu uptake in rat cerebral cortex are increased by phencyclidine. Glia 56: 1320-1327.

Bragina L, Melone M, Fattorini G, Conti F (2007) Clozapine up-regulates the expression of the vesicular GABA transporter (VGAT) in rat frontal cortex. Molecular Psychiatry 12: 612-613.

Bragina L, Melone M, Fattorini G, Torres-Ramos M, Vallejo-Illarramendi A, Matute C, Conti F (2006) GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation. Journal of Neurochemistry 99:134-141.

Melone M, Varoqui H, Erickson JD, Conti F (2006) Localization of the amino acid transporter SNAT2 in the cerebral cortex. Neuroscience, 140: 281-292.

Conti F, Melone M (2006) The glutamine commute: lost in the tube? Neurochemistry International 48: 459-464.

Melone M, Burette A, Weinberg RJ (2005) Light microscopic identification and immunocytochemical characterization of glutamatergic synapses in brain sections. Journal of Comparative Neurology 492: 495-509.

Melone M, Barbaresi P, Fattorini G, Conti F (2005) Neuronal localization of the GABA transporter GAT-3 in human cerebral cortex: A procedural artifact? Journal of Chemical Neuroanatomy 30: 45-54.

Vallejo-Illarramendi A, Torres-Ramos M, Melone M, Conti F, Matute C (2005) Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures. Glia 50: 276-279.

Matute C, Melone M, Vallejo-Illarramendi A, Conti F (2005) Increased expression of the astrocytic glutamate transporter GLT-1 in the prefrontal cortex of schizophrenics. Glia 49: 451-455.

Conti F, Minelli A, Melone M (2004) GABA transporters in the mammalian cerebral cortex: localization, development and pathological implications. Brain Research/Brain Research Review 45: 196-212.

Melone M, Quagliano F, Barbaresi P, Varoqui H, Erickson JD, Conti F (2004) Localization of the glutamine transporter SNAT1 in rat cerebral cortex and neighboring structures, with a note on its localization in human cortex. Cerebral Cortex 14: 562-574.

Alonso-Nanclares L, Minelli A, Melone M, Edwards RH, DeFelipe J, Conti F (2004) Perisomatic glutamatergic axon terminals: a novel feature of cortical synaptology revealed by vesicular glutamate transporter 1 immunostaining. Neuroscience 123: 547-556.

Melone M, Cozzi A, Pellegrini-Giampietro DE, Conti F (2003) Transient focal ischemia triggers neuronal expression of GAT-3 in the rat perilesional cortex. Neurobiology of Disease 14: 120-132.

Melone M, Bragina L, Conti F (2003) Clozapine-induced reduction of glutamate transport in the frontal cortex is not mediated by GLAST and EAAC1. Molecular Psychiatry 8: 12-13.

Melone M, Vitellaro Zuccarello L, A Vallejo-Illarramendi, A Perez-Samartin, C Matute, A Cozzi, DE Pellegrini-Giampietro, JD Rothstein, Conti F (2001) The expression of glutamate transporter GLT-1 in the cerebral cortex is down-regulated by the antipsychotic clozapine. Molecular Psychiatry 6: 380-386.

Melone M, Brecha N, Sternini C, Evans C, and Conti F (2000) Etorphine increases the number of µ-opioid receptor-positive cells in the cerebral cortex. Neuroscience 100: 439-443.

Conti F, Vitellaro-Zuccarello L, Barbaresi P, Minelli A, Brecha NC, and M. Melone (1999) Neuronal, glial, and epithelial localization of γ-aminobutyric acid transporter-2, a high-affinity γ-aminobutyric acid plasma membrane transporter, in the cerebral cortex and neighboring structures. Journal of Comparative Neurology 409: 482-494.

Conti F, Barbaresi P, Melone M, and Ducati A (1999) Neuronal and glial localization of NR1 and NR2A/B subunits of the NMDA receptor in the human cerebral cortex. Cerebral Cortex 9: 110-120.

Conti F, Melone M, De Biasi S, Ducati A, Minelli A, and Brecha NC (1998) Neuronal and glial localization of GAT-1, a high-affinity GABA plasma membrane transporter, in the human cerebral cortex. Journal of Comparative Neurology 396: 51-63.

Conti F, De Biasi S, Minelli A, Rothstein JD, and Melone M (1998) EAAC1, a high-affinity glutamate transporter, is localized to neurons and astrocytes in the cerebral cortex. Cerebral Cortex 8: 108-116.

DeBiasi S, Minelli A, Melone M, and F Conti (1996) Presynaptic NMDA receptors in the rat cerebral cortex are both auto- and heteroreceptors. NeuroReport 7: 2773-2776.

Conti F, De Biasi S, Minelli A, and Melone M (1996) Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes. Glia 17: 254-258.